Fluorescence Spectrum

On this page you can find examples of different fluorescence spectra measured using our PEBBLE VIS full spectrum spectrometer. Each fluorescence spectrum corresponds to a particular fluorophore.

Measurement set-up

The set-up used to make the measurements is shown on the figure below. The excitation light from the LED is passed through a short pass (SP) filter and directed to the sample by a dichroic filter. The fluorescent light from the sample is passed through the dichroic filter and a long pass (LP) filter and detected by a PEBBLE VIS ultra compact spectrometer. This spectrophotometer covers 380 – 850 nm with a resolution of 6 nm. The wavelength range is sampled by 256 pixels.

The set-up used to make the measurements for different fluorescence spectra using the PEBBLE VIS spectrometer

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Fluorescence Spectrum of Chlorophyll

Graph of captured fluorescence spectrum of Chlorophyll in a leaf

The fluorescent spectrum below is recording with a white LED as light source and a leaf from one of our office plants as sample. The graph shows a nice cholyphyll fluorescence spectrum with a peak at 673 – 679 nm.

Fluid Fluorophores

Fluoroscence spectra of Fluorescein, Rhodamine, ADOTA, Cresyl Violet and DMQA using the PEBBLE VIS spectrometer

The following measurements have been obtained at The Department of Chemistry, Copenhagen University with the help of Dr. Thomas Just Sørensen.  All measurements were captured using the PEBBLE VIS spectrometer connected to a fluorescence front-end optics with a 405 nm LED, a 455 nm short pass excitation filter,  a  455 nm dichroic filter and a 466 nm Long Pass Emission filter. The integration time was around 100 msec for Fluorescein and Rhodamine, ADOTA  and 500 msec for Cresyl Violet and DMQA.

Measuring Fluid Fluorophores with our PEBBLE Spectrometer