High Pressure Liquid Chromatography

High Pressure Liquid Chromatography (HPLC) is a form of chromatography in which the liquid mobile phase is forced through a column of solid stationary phase under high pressure. HPLC is a powerful analytical tool used to purify, separate, identify, and quantify each component in a mixture.

High Pressure Liquid Chromatography Illustration

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An HPLC instrument consists of a mobile phase, a pump, an injector, a separation column, and a detector. The sample material is analyzed by pumping a liquid phase (solvent) mixed with the sample through a column packed with a solid phase material. Each component in the mixture responds differently to the absorbent material and causing the constituents of the sample material to separate and come out of the column at different times. Out comes first the material that binds well to the liquid phase and secondly, the material that binds well to the solid phase. HPLC is used to measure the content of various constituents of sample material or for purification. As an example, it can be used in the pharma industry, where HPLC is very useful in measuring the level of active drug in a given material.

Illustration of High Pressure Liquid Chromatography Setup

Almost 90% of all HPLC systems use an UV-detector to measure the concentration through absorbance spectroscopy. The concentration is found through the Beer-Lambert’s Law: A = ε c L. The detector measures the absorbance versus time at one or more wavelengths. There are three types of detectors. A single wavelength detector with a fixed optical filter, a selectable wavelength detector with a rotating grating and a multiple wavelength Diode-Array-Detector (DAD). Ibsen offer DAD spectrometers which are the fastest and most versatile form of spectrometer with UV-detectors.

Detectors for HPLC

Ibsen has a number of OEM spectrometer platforms available, that are well suited for integration into your high pressure liquid chromatography analysis system.

Here you can read why especially our FREEDOM UV-VIS spectrometer is well suited to be integrated into a HPLC instrument.

If you need a better resolution, another wavelength, or if none of the examples fit your specific needs for other reasons, we can always discuss a customized spectrometer to fit your requirements.


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Below is a short list of spectrometer examples

190 - 850 nm
190 - 1100 nm
190 - 435 nm
360 - 830 nm