A fluorometer, also known as a spectrofluorometer, is an instrument that is capable of measuring the optical fluorescence from one or more fluorophores. On this page, we illustrate the basics of a fluorometer design.
You can learn more about the basics of fluorescence spectroscopy by clicking here or watch our video tutorial here..
There are many types and names for instruments capable of measuring fluorescence, like fluorometer, fluorimeter, fluorescence spectrophotometer, and micro plate reader. You can get a more detailed description here. A fluorometer is generally used to describe an instrument with a dedicated application in mind such as chorophyll measurements.
The function of a fluorometer is to illuminate the sample with light at a specific wavelength and measure the emitted fluorescent light from the sample at one or more wavelengths. The figure below shows the basic construction of a fluorometer.
A fluorometer consists of the following basic components:
Below, the various components are described in more detail.