Fluorescence spectroscopy

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Fluorescence Spectroscopy

Fluorescence is a process in which a molecule, quantum dot or other quantum emitter gets into an excited state by absorbing light and in return emits light of a different color. Because some energy is lost in the time the molecule is in the excited state, generally the emitted light is red-shifted with respect to the excitation wavelength. Fluorescence is used widely in biology to image the interior of organisms and spectroscopy can give valuable information on the physical conditions in the cell or allow for multiplexing.

FluorescenceSpectra
Watch our webinar “Basics of Fluorescence Spectroscopy”

Fluorescence spectrometer products

A number of OEM spectrometers are available at Ibsen that is well suited for fluorescence spectroscopy and measurements.

We recommend the following fluorescence spectrometer options:

Ultra-compact PEBBLE VIS and the Compact FREEDOM spectrometers being cost-efficient with high performance and our High Throughput ROCK spectrometers for their uncompromised performance.

Our transmission grating based spectrometers are ideally suited for fluorescence spectroscopy due to the following benefits:

  • Nearly 100% polarization independent efficiency (optical throughput)

  • Integration with a range of common CCD and CMOS detectors

  • Robustness for portability, usage, or challenging industrial environments

  • Low temperature dependence

  • Compact size

Fluorescence-with-Freedom

Fluorescence instrumentation

Fluorescence spectrum

How to build a fluorometer

Flow cytometer

DDA vs filters

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For further information see below.

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